BIOSPERATIONS: PRINCIPLES AND TECHNIQUESThis systematically organized and well-balanced book compresses within the covers of a single volume the theoretical principles and techniques involved in bio-separations, also called downstream processing. These techniques are derived from a range of subjects, for example, physical chemistry, analytical chemistry, bio-chemistry, biological science and chemical engineering. Organized in its 15 chapters, the text covers in the first few chapters topics related to chemical engineering unit operations such as filtration, centrifugation, adsorption, extraction and membrane separation as applied to bioseparations. The use of chromatography as practiced at laboratory as well as industrial scale operation and related techniques such as gel filtration, affinity and pseudoaffinity chromatography, ion-exchange chromatography, electrophoresis and related methods have been discussed. The important applications of these techniques have also been highlighted. |
Contents
CELL DISRUPTION 1325 | 13 |
FILTRATION 2641 | 26 |
CENTRIFUGATION 4253 | 42 |
ADSORPTION 5466 | 54 |
EXTRACTION 6 7 99 | 67 |
MEMBRANE SEPARATION PROCESSES 100118 | 100 |
PRECIPITATION | 119 |
Exercises | 141 |
Exercises | 171 |
Exercises | 186 |
Questions | 199 |
12 | 200 |
ELECTROKINETIC METHODS OF SEPARATION 240254 | 240 |
Questions | 254 |
Questions | 265 |
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Common terms and phrases
acid adsorbent adsorption affinity agarose amino anions aqueous two-phase batch binding bioseparations buffer cake capacity cell disruption cell wall centrifuge charge chemical chromatographic techniques chromatography column constant containing covalent crystals decreases denaturation density depends desired product desired protein detergents dextran diffusion downstream processing efficiency electrophoresis eluent elution enzymes equation equilibrated equilibrium ethanol extraction factor feed fermentation broths field filter medium first flow flow rate gel filtration given by Eq hence hydrophobic interaction immobilized increases influence involves ion exchanger ionic strength isoelectric isoelectric points layer ligand liquid mass transfer matrix metal ion method mixture mobile phase molecular weight molecules organic solvent osmotic particles partition coefficient peak plates polarity polymers pores purification retention salt sample selectivity shown in Figure solids solubility solute components solute concentration specific stationary phase sulphate supercritical fluid surface temperature theoretical plates thiol values velocity viscosity volume washing yeast